Review



cytochrome c oxidase subunit 4  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc cytochrome c oxidase subunit 4
    Cytochrome C Oxidase Subunit 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/COX+IV+(4D11-B3-E8)+Mouse+mAb/pmc12816905-99-54-60
    Average 96 stars, based on 221 article reviews
    cytochrome c oxidase subunit 4 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-HA-Tag (Cell signaling Technology, Cat# 3724) is validated for Western blot analysis, immunoprecipitation, immunohistochemical analysis, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for cells containing HA-tagged protein (https://www.cellsignal.com/products/primary-antibodies/ha-tag-c29f4-rabbitmab/3724). .. Mouse anti-"-actin (Cell signaling Technology, Cat# 3700) is validated for Western blot analysis, immunohistochemical analysis, immunofluorescence analysis, and flow cytometric analysis; this antibody works for human, mouse, rat, hamster, monkey, and dog (https://www.cellsignal.com/products/primary-antibodies/b-actin-8h10d10-mouse-mab/3700). .. Mouse anti-GST (Santa Cruz Biotechnology, Cat# sc-138) is validated for Western blot analysis and immunoprecipitation; this antibody works for GST fusion proteins of Schistosoma japonicum origin and recombinant GST fusion proteins expressed in E. coli (specifically designed to be used in combination with GST expression vectors such as pGEX.3X and pGEX.2T) (https://www.scbt.com/ p/gst-antibody-b-14).

    Article Title: Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models
    Article Snippet: .. Primary Antibodies for Histology anti-OCN (ab93876, Abcam), anti-COX2 (ab15191, Abcam), anti-CD68 (ab125212, ab31630, Abcam), anti-CGRP (ab81887, Abcam), anti-PGP9.5 (ab108986), anti-SP7 (ab22552, Abcam), anti-RUNX2 (ab76956, abcam), anti-Tyrosine Hydroxylase (AB152, Merck Millipore), anti-Phospho-CREB (ab32096, abcam), anti-CREB (ab32515, abcam), anti-CD11c (#97585, CST), anti-F4/80 (#70076, CST), anti-CD19 (#90176, CST), anti-CD3 (#99940, CST) Primary Antibodies for Western Blotting anti- NF-$B p65 (#8241, CST), anti-Phospho-NF-$B p65 (#3033, CST), anti-Phospho-I$B" (#2859, CST), anti-I$B" (#4814, CST), antiHTR2C (ab197776, abcam), mouse anti-#-actin (#8457, CST) Secondary Antibodies for Histology Alexa-Fluor 488-conjugated secondary antibody (A11008, ThermoFisher), Alexa-Fluor 647-conjugated secondary antibody (A21240, ThermoFisher) Histology anti-OCN (ab93876, Abcam) was validated for IHC staining of mouse sample. ..

    Immunohistochemical staining:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-HA-Tag (Cell signaling Technology, Cat# 3724) is validated for Western blot analysis, immunoprecipitation, immunohistochemical analysis, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for cells containing HA-tagged protein (https://www.cellsignal.com/products/primary-antibodies/ha-tag-c29f4-rabbitmab/3724). .. Mouse anti-"-actin (Cell signaling Technology, Cat# 3700) is validated for Western blot analysis, immunohistochemical analysis, immunofluorescence analysis, and flow cytometric analysis; this antibody works for human, mouse, rat, hamster, monkey, and dog (https://www.cellsignal.com/products/primary-antibodies/b-actin-8h10d10-mouse-mab/3700). .. Mouse anti-GST (Santa Cruz Biotechnology, Cat# sc-138) is validated for Western blot analysis and immunoprecipitation; this antibody works for GST fusion proteins of Schistosoma japonicum origin and recombinant GST fusion proteins expressed in E. coli (specifically designed to be used in combination with GST expression vectors such as pGEX.3X and pGEX.2T) (https://www.scbt.com/ p/gst-antibody-b-14).

    Immunofluorescence:

    Article Title: Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation
    Article Snippet: Rabbit anti-HA-Tag (Cell signaling Technology, Cat# 3724) is validated for Western blot analysis, immunoprecipitation, immunohistochemical analysis, immunofluorescence analysis, flow cytometric analysis, and chromatin immunoprecipitation; this antibody works for cells containing HA-tagged protein (https://www.cellsignal.com/products/primary-antibodies/ha-tag-c29f4-rabbitmab/3724). .. Mouse anti-"-actin (Cell signaling Technology, Cat# 3700) is validated for Western blot analysis, immunohistochemical analysis, immunofluorescence analysis, and flow cytometric analysis; this antibody works for human, mouse, rat, hamster, monkey, and dog (https://www.cellsignal.com/products/primary-antibodies/b-actin-8h10d10-mouse-mab/3700). .. Mouse anti-GST (Santa Cruz Biotechnology, Cat# sc-138) is validated for Western blot analysis and immunoprecipitation; this antibody works for GST fusion proteins of Schistosoma japonicum origin and recombinant GST fusion proteins expressed in E. coli (specifically designed to be used in combination with GST expression vectors such as pGEX.3X and pGEX.2T) (https://www.scbt.com/ p/gst-antibody-b-14).

    Incubation:

    Article Title: Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.
    Article Snippet: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China Laboratory Animal Research Center for Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China Formula-Pattern Research Center, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China College of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China

    Binding Assay:

    Article Title: Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.
    Article Snippet: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China Laboratory Animal Research Center for Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China Formula-Pattern Research Center, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China College of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, People's Republic of China

    other:

    Article Title: Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling
    Article Snippet: The investigators were blinded to group allocation during data collection and analysis. anti-ACP5 (Gentex GTX100438, CA, USA, 1:1,000); mouse anti-ACP5 (Abnova H00000054-M01, Taipei, China, 1: 100), rabbit anti-ACP5 (Proteintech 11594-1-AP, Wuhan, China, 1:100), rabbit anti-FSP1 (Proteintech 16105-1-AP Wuhan, China, 1:100), anti-LAMIN B1(Proteintech 12987-1-AP, Wuhan, China, 1:1,000), anti-FIBRONECTIN (Proteintech 15613-1-AP, Wuhan, China, 1:1,000) anti-COL1A1 (Proteintech 67288-1-lg, Wuhan, China, 1:1,000) anti-!-SMA(Cell Signaling Technology 19245, MA, USA, 1: 1000), anti-phospho-"-CATENIN (Ser33/37/Thr41) (Cell Signaling Technology 9561, MA, USA, 1: 1000), anti-"-CATENIN (Cell Signaling Technology 8480, MA, USA, 1: 1000); anti-Pcna (Cell Signaling Technology 13110, MA, USA, 1:100) mouse anti-!-SMA (Cell Signaling Technology 48938, MA, USA, 1:100), rabbit anti-phospho-smad3 (Cell Signaling Technology 9520, MA, USA, 1:100). anti-GAPDH(Abcam ab8245, MA, USA, 1:3000). anti-"-ACTIN (Abcam ab8226, MA, USA, 1:3000).

    Chromatin Immunoprecipitation:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants primary antibodies: mouse anti-GFP (RT0265, Bio X Cell; Lebanon, NH, 1:2000), rabbit anti-Ras (3965, Cell Signaling Technology; Danvers, MA, 1:1000), rabbit anti-SAPK/JNK (9252, Cell Signaling Technology, 1:2000), rabbit anti-!-tubulin (2144, Cell Signaling Technology, 1:2000), rabbit anti-Erk1/2 (9102, Cell Signaling Technology, 1:1000), rabbit anti-Bcl-xL (2764, Cell Signaling Technology, 1:1000), and mouse anti-"actin (3700, Cell Signaling Technology, 1:4000). mouse anti-phospho-Erk1/2 (9106, Cell Signaling Technology, 1:1000). secondary antibodies: goat anti-rabbit 680RD (925-68071, LI-COR, 1:15,000), donkey anti-mouse 800CW (925-32212, LI-COR, 1:15,000) all CST antibodies were validated by the manufacture with data presented on the website. ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Primary antibodies mouse anti-HA (1:1,000, #901533, Biolegend) mouse anti-!-Tubulin (1:1,000, #5886, Cell Signalling) rabbit anti-GFP (1:1,000, #2555, Cell Signalling) rabbit anti-53BP1 (1:800, #A300-272A, Bethyl laboratories) rabbit anti-RAD51 (1:1,000, #70-012, Bioacademia) rat anti-BrdU (CldU) (1:250, #ab6326, Abcam) mouse anti-BrdU (IdU) (1:40, #347580, BD Biosciences) rabbit anti-CtIP (1:500, #A300-487A, Bethyl Laboratories) mouse anti-Biotin (1:200, #200-002-211, Jackson Immuno Research) rabbit anti-MRE11 (1:500, a kind gift from Arnab Ray Chaudhuri) rabbit anti-H2AX (1:1000, #NB100-383, Novus Biologicals) Secondary antibodies anti-rabbit Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) anti-mouse Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) Alexa Fluor 488 goat anti-mouse (#A-11029, ThermoFisher Scientific, 1:600) Cy3 AffiniPure F(ab')" fragment donkey anti-rat (#712-165-513, Jackson Immuno Research, 1:150) Alexa Fluor 568 goat anti-rabbit (1:2,000, # T-6391, ThermoFisher Scientific) All antibodies used are commercially available and have been previously validated by the corresponding manufacturers as stated in their websites. ..

    Flow Cytometry:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants primary antibodies: mouse anti-GFP (RT0265, Bio X Cell; Lebanon, NH, 1:2000), rabbit anti-Ras (3965, Cell Signaling Technology; Danvers, MA, 1:1000), rabbit anti-SAPK/JNK (9252, Cell Signaling Technology, 1:2000), rabbit anti-!-tubulin (2144, Cell Signaling Technology, 1:2000), rabbit anti-Erk1/2 (9102, Cell Signaling Technology, 1:1000), rabbit anti-Bcl-xL (2764, Cell Signaling Technology, 1:1000), and mouse anti-"actin (3700, Cell Signaling Technology, 1:4000). mouse anti-phospho-Erk1/2 (9106, Cell Signaling Technology, 1:1000). secondary antibodies: goat anti-rabbit 680RD (925-68071, LI-COR, 1:15,000), donkey anti-mouse 800CW (925-32212, LI-COR, 1:15,000) all CST antibodies were validated by the manufacture with data presented on the website. ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Primary antibodies mouse anti-HA (1:1,000, #901533, Biolegend) mouse anti-!-Tubulin (1:1,000, #5886, Cell Signalling) rabbit anti-GFP (1:1,000, #2555, Cell Signalling) rabbit anti-53BP1 (1:800, #A300-272A, Bethyl laboratories) rabbit anti-RAD51 (1:1,000, #70-012, Bioacademia) rat anti-BrdU (CldU) (1:250, #ab6326, Abcam) mouse anti-BrdU (IdU) (1:40, #347580, BD Biosciences) rabbit anti-CtIP (1:500, #A300-487A, Bethyl Laboratories) mouse anti-Biotin (1:200, #200-002-211, Jackson Immuno Research) rabbit anti-MRE11 (1:500, a kind gift from Arnab Ray Chaudhuri) rabbit anti-H2AX (1:1000, #NB100-383, Novus Biologicals) Secondary antibodies anti-rabbit Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) anti-mouse Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) Alexa Fluor 488 goat anti-mouse (#A-11029, ThermoFisher Scientific, 1:600) Cy3 AffiniPure F(ab')" fragment donkey anti-rat (#712-165-513, Jackson Immuno Research, 1:150) Alexa Fluor 568 goat anti-rabbit (1:2,000, # T-6391, ThermoFisher Scientific) All antibodies used are commercially available and have been previously validated by the corresponding manufacturers as stated in their websites. ..

    Magnetic Resonance Imaging:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants primary antibodies: mouse anti-GFP (RT0265, Bio X Cell; Lebanon, NH, 1:2000), rabbit anti-Ras (3965, Cell Signaling Technology; Danvers, MA, 1:1000), rabbit anti-SAPK/JNK (9252, Cell Signaling Technology, 1:2000), rabbit anti-!-tubulin (2144, Cell Signaling Technology, 1:2000), rabbit anti-Erk1/2 (9102, Cell Signaling Technology, 1:1000), rabbit anti-Bcl-xL (2764, Cell Signaling Technology, 1:1000), and mouse anti-"actin (3700, Cell Signaling Technology, 1:4000). mouse anti-phospho-Erk1/2 (9106, Cell Signaling Technology, 1:1000). secondary antibodies: goat anti-rabbit 680RD (925-68071, LI-COR, 1:15,000), donkey anti-mouse 800CW (925-32212, LI-COR, 1:15,000) all CST antibodies were validated by the manufacture with data presented on the website. ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Primary antibodies mouse anti-HA (1:1,000, #901533, Biolegend) mouse anti-!-Tubulin (1:1,000, #5886, Cell Signalling) rabbit anti-GFP (1:1,000, #2555, Cell Signalling) rabbit anti-53BP1 (1:800, #A300-272A, Bethyl laboratories) rabbit anti-RAD51 (1:1,000, #70-012, Bioacademia) rat anti-BrdU (CldU) (1:250, #ab6326, Abcam) mouse anti-BrdU (IdU) (1:40, #347580, BD Biosciences) rabbit anti-CtIP (1:500, #A300-487A, Bethyl Laboratories) mouse anti-Biotin (1:200, #200-002-211, Jackson Immuno Research) rabbit anti-MRE11 (1:500, a kind gift from Arnab Ray Chaudhuri) rabbit anti-H2AX (1:1000, #NB100-383, Novus Biologicals) Secondary antibodies anti-rabbit Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) anti-mouse Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) Alexa Fluor 488 goat anti-mouse (#A-11029, ThermoFisher Scientific, 1:600) Cy3 AffiniPure F(ab')" fragment donkey anti-rat (#712-165-513, Jackson Immuno Research, 1:150) Alexa Fluor 568 goat anti-rabbit (1:2,000, # T-6391, ThermoFisher Scientific) All antibodies used are commercially available and have been previously validated by the corresponding manufacturers as stated in their websites. ..

    Biomarker Discovery:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Novel plant genotypes Seed stocks Authentication Plants primary antibodies: mouse anti-GFP (RT0265, Bio X Cell; Lebanon, NH, 1:2000), rabbit anti-Ras (3965, Cell Signaling Technology; Danvers, MA, 1:1000), rabbit anti-SAPK/JNK (9252, Cell Signaling Technology, 1:2000), rabbit anti-!-tubulin (2144, Cell Signaling Technology, 1:2000), rabbit anti-Erk1/2 (9102, Cell Signaling Technology, 1:1000), rabbit anti-Bcl-xL (2764, Cell Signaling Technology, 1:1000), and mouse anti-"actin (3700, Cell Signaling Technology, 1:4000). mouse anti-phospho-Erk1/2 (9106, Cell Signaling Technology, 1:1000). secondary antibodies: goat anti-rabbit 680RD (925-68071, LI-COR, 1:15,000), donkey anti-mouse 800CW (925-32212, LI-COR, 1:15,000) all CST antibodies were validated by the manufacture with data presented on the website. ..

    Article Title: H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Primary antibodies mouse anti-HA (1:1,000, #901533, Biolegend) mouse anti-!-Tubulin (1:1,000, #5886, Cell Signalling) rabbit anti-GFP (1:1,000, #2555, Cell Signalling) rabbit anti-53BP1 (1:800, #A300-272A, Bethyl laboratories) rabbit anti-RAD51 (1:1,000, #70-012, Bioacademia) rat anti-BrdU (CldU) (1:250, #ab6326, Abcam) mouse anti-BrdU (IdU) (1:40, #347580, BD Biosciences) rabbit anti-CtIP (1:500, #A300-487A, Bethyl Laboratories) mouse anti-Biotin (1:200, #200-002-211, Jackson Immuno Research) rabbit anti-MRE11 (1:500, a kind gift from Arnab Ray Chaudhuri) rabbit anti-H2AX (1:1000, #NB100-383, Novus Biologicals) Secondary antibodies anti-rabbit Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) anti-mouse Horseradish Peroxidase (HRP)-linked secondary antibodies (1: 5,000, Cell Signalling) Alexa Fluor 488 goat anti-mouse (#A-11029, ThermoFisher Scientific, 1:600) Cy3 AffiniPure F(ab')" fragment donkey anti-rat (#712-165-513, Jackson Immuno Research, 1:150) Alexa Fluor 568 goat anti-rabbit (1:2,000, # T-6391, ThermoFisher Scientific) All antibodies used are commercially available and have been previously validated by the corresponding manufacturers as stated in their websites. ..

    Immunohistochemistry:

    Article Title: Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models
    Article Snippet: .. Primary Antibodies for Histology anti-OCN (ab93876, Abcam), anti-COX2 (ab15191, Abcam), anti-CD68 (ab125212, ab31630, Abcam), anti-CGRP (ab81887, Abcam), anti-PGP9.5 (ab108986), anti-SP7 (ab22552, Abcam), anti-RUNX2 (ab76956, abcam), anti-Tyrosine Hydroxylase (AB152, Merck Millipore), anti-Phospho-CREB (ab32096, abcam), anti-CREB (ab32515, abcam), anti-CD11c (#97585, CST), anti-F4/80 (#70076, CST), anti-CD19 (#90176, CST), anti-CD3 (#99940, CST) Primary Antibodies for Western Blotting anti- NF-$B p65 (#8241, CST), anti-Phospho-NF-$B p65 (#3033, CST), anti-Phospho-I$B" (#2859, CST), anti-I$B" (#4814, CST), antiHTR2C (ab197776, abcam), mouse anti-#-actin (#8457, CST) Secondary Antibodies for Histology Alexa-Fluor 488-conjugated secondary antibody (A11008, ThermoFisher), Alexa-Fluor 647-conjugated secondary antibody (A21240, ThermoFisher) Histology anti-OCN (ab93876, Abcam) was validated for IHC staining of mouse sample. ..

    Staining:

    Article Title: Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models
    Article Snippet: .. Primary Antibodies for Histology anti-OCN (ab93876, Abcam), anti-COX2 (ab15191, Abcam), anti-CD68 (ab125212, ab31630, Abcam), anti-CGRP (ab81887, Abcam), anti-PGP9.5 (ab108986), anti-SP7 (ab22552, Abcam), anti-RUNX2 (ab76956, abcam), anti-Tyrosine Hydroxylase (AB152, Merck Millipore), anti-Phospho-CREB (ab32096, abcam), anti-CREB (ab32515, abcam), anti-CD11c (#97585, CST), anti-F4/80 (#70076, CST), anti-CD19 (#90176, CST), anti-CD3 (#99940, CST) Primary Antibodies for Western Blotting anti- NF-$B p65 (#8241, CST), anti-Phospho-NF-$B p65 (#3033, CST), anti-Phospho-I$B" (#2859, CST), anti-I$B" (#4814, CST), antiHTR2C (ab197776, abcam), mouse anti-#-actin (#8457, CST) Secondary Antibodies for Histology Alexa-Fluor 488-conjugated secondary antibody (A11008, ThermoFisher), Alexa-Fluor 647-conjugated secondary antibody (A21240, ThermoFisher) Histology anti-OCN (ab93876, Abcam) was validated for IHC staining of mouse sample. ..



    Similar Products

    99
    LI-COR anti mouse ir680
    Anti Mouse Ir680, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/GOATaRABBIT+IRDye+800CW/pmc12816905-100-7-14
    Average 99 stars, based on 1 article reviews
    anti mouse ir680 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    Bio-Rad rat anti mouse monoclonal f4 80
    Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/Rat+anti+Mouse+F4%2F80/10__22203_slash_ecm__v033a11-129-8-16
    Average 96 stars, based on 1 article reviews
    rat anti mouse monoclonal f4 80 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad goat anti mouse igg
    Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/Goat+anti+Mouse+IgG/pmc13004575-85-30-34
    Average 96 stars, based on 1 article reviews
    goat anti mouse igg - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc cytochrome c oxidase subunit 4
    Cytochrome C Oxidase Subunit 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/COX+IV+(4D11-B3-E8)+Mouse+mAb/pmc12816905-99-54-60
    Average 96 stars, based on 1 article reviews
    cytochrome c oxidase subunit 4 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad horse radish peroxidase hrp secondary antibody
    Horse Radish Peroxidase Hrp Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/Goat+anti+Mouse+IgG+(H%2FL)/pmc12828604-98-7-22
    Average 96 stars, based on 1 article reviews
    horse radish peroxidase hrp secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Arigo Biolaboratories mouse anti cd68
    Mouse Anti Cd68, supplied by Arigo Biolaboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/anti+cd68+mouse/pmc13273865-449-6-13
    Average 86 stars, based on 1 article reviews
    mouse anti cd68 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Coralite Dental Products rabbit anti mouse mhc i antibody
    Rabbit Anti Mouse Mhc I Antibody, supplied by Coralite Dental Products, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/anti+igg+rabbit/pmc13191106-419-18-10
    Average 86 stars, based on 1 article reviews
    rabbit anti mouse mhc i antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Coralite Dental Products rat coralite plus 488 anti mouse lamp1 antibody
    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
    Rat Coralite Plus 488 Anti Mouse Lamp1 Antibody, supplied by Coralite Dental Products, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/488+anti+coralite+lamp1+mouse+plus+rat/pmc13191106-394-46-47
    Average 86 stars, based on 1 article reviews
    rat coralite plus 488 anti mouse lamp1 antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Servicebio Inc anti ki67 mouse mab
    In vivo therapeutic efficacy of advanced BAITs in a postoperative tumor model. (A) Schematic of in vivo experimental design. LLC tumors were surgically resected, and mice were re-challenged with LLC cells. Mice then received BAIT treatments. (B) Photographs of tumors and tumor weights at day 16 for each group (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗ is P < 0.001 by one-way ANOVA with Bonferroni post-hoc test). (C) Individual tumor growth curves over time (n = 5; ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (D) H&E-stained tumor sections. (E) TUNEL staining of tumor sections (brown, apoptotic cells). Black arrowheads mark TUNEL + areas. (F) Quantification of TUNEL + apoptotic cells (n = 5; ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (G) Representative immunofluorescence for <t>Ki67</t> (green) in tumor tissues (nuclei in blue). The Apo-BAIT group shows greatly reduced Ki67 + proliferating cells. (H) Quantification of Ki67 + cell density (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). Data are presented as mean ± SD.
    Anti Ki67 Mouse Mab, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti/anti+ki67+%E2%80%A2/pmc13191106-322-0-42
    Average 86 stars, based on 1 article reviews
    anti ki67 mouse mab - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

    In vivo therapeutic efficacy of advanced BAITs in a postoperative tumor model. (A) Schematic of in vivo experimental design. LLC tumors were surgically resected, and mice were re-challenged with LLC cells. Mice then received BAIT treatments. (B) Photographs of tumors and tumor weights at day 16 for each group (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗ is P < 0.001 by one-way ANOVA with Bonferroni post-hoc test). (C) Individual tumor growth curves over time (n = 5; ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (D) H&E-stained tumor sections. (E) TUNEL staining of tumor sections (brown, apoptotic cells). Black arrowheads mark TUNEL + areas. (F) Quantification of TUNEL + apoptotic cells (n = 5; ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (G) Representative immunofluorescence for Ki67 (green) in tumor tissues (nuclei in blue). The Apo-BAIT group shows greatly reduced Ki67 + proliferating cells. (H) Quantification of Ki67 + cell density (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: In vivo therapeutic efficacy of advanced BAITs in a postoperative tumor model. (A) Schematic of in vivo experimental design. LLC tumors were surgically resected, and mice were re-challenged with LLC cells. Mice then received BAIT treatments. (B) Photographs of tumors and tumor weights at day 16 for each group (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗ is P < 0.001 by one-way ANOVA with Bonferroni post-hoc test). (C) Individual tumor growth curves over time (n = 5; ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (D) H&E-stained tumor sections. (E) TUNEL staining of tumor sections (brown, apoptotic cells). Black arrowheads mark TUNEL + areas. (F) Quantification of TUNEL + apoptotic cells (n = 5; ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (G) Representative immunofluorescence for Ki67 (green) in tumor tissues (nuclei in blue). The Apo-BAIT group shows greatly reduced Ki67 + proliferating cells. (H) Quantification of Ki67 + cell density (n = 5; ∗ is P < 0.05, ∗∗ is P < 0.01, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). Data are presented as mean ± SD.

    Article Snippet: Anti-Ki67 mouse mAb (Cat# GB121141-100), anti-CD3 mouse mAb (Cat# GB15014-100), FITC-conjugated goat anti-mouse IgG (H + L) (Cat# GB22301), and Cy5-conjugated goat anti-mouse IgG (H + L) (Cat# GB27301) for immunofluorescence assays, and DAB (SA-HRP) TUNEL apoptosis detection kit were supplied by Servicebio (Wuhan, China).

    Techniques: In Vivo, Drug discovery, Staining, TUNEL Assay, Immunofluorescence